Lourdes and Gloria have always had a very close relationship. She even showed up to Gloria's ultrasound sessions when the father of Gloria's children was clearly absent ("Trust No Bitch").
Lourdes is first referred to in "Looks Blue, Tastes Red" when Gloria mentions she has an aunt who is a "santera". Gloria says later that Lourdes refers to her magic as "Catholic plus".
Lourdes is then seen practicing her magic on a customer at the convenience store Gloria works at. Later, when Arturo becomes abusive with Gloria, Lourdes steps in and defends her niece. Upon Gloria's arrest for food stamp fraud, Lourdes takes in her great-nephews, Benito and Julio ("Low Self Esteem City").
Some time after Gloria's arrest, Lourdes moves to Florida with her great-nephews. Years later, in "Mother's Day", Gloria learns that the family will be moving back to New York City, allowing Gloria to visit with them in person.